缺氧誘導(dǎo)因子1α抑制劑對人卵巢癌移植瘤生長的抑制作用.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文F773089缺氧誘導(dǎo)因子IⅨ抑制劑對人卵巢癌移植瘤生長的抑制作用Hypoxiainduciblefactor(HIF—1)ainhibitorcorrelatedwithtumourgrowthinovarianepithelialcallcermode碩上研究生導(dǎo)師蔣紅元豐有吉教授導(dǎo)師小組成員周先榮教授學(xué)科專業(yè)院系姚明研究員婦產(chǎn)科學(xué)、婦科腫瘤婦產(chǎn)科醫(yī)院2004年8爿未鐮零息勿壘又公布“AbstractObject

2、ivesToinvestigate，amTORinhibitor，rapamycin，inhibitedexpressionofHIF一1oproteinandtumorgrowthinxenogeneiCtransplantmodelofovariancancerAndinvestigatethecorrelationofHIF—IⅡ，VEGFandapoptosisMethods1Femalenudemieewereinoeufat

3、edsubcutaneouswithSKOV一3cellSLater，miceweretreatedwithsaline，rapamycin，paclitaxelorrapomycinpac]itaxe]Tumorburdenofmiceineachgroupwereevaluated2TheexpressionsofHIF一1Ⅱ，VEGFandMVDwereexaminedbyimmunohistochemistryThemRNAso

4、fGlutlandVEGFwerestudiedbyusingofRT—PCR3ApoptosiSoftumorcellSwasdeterminedbyTUNELaswellasbcl一2mRNAwasdeterminedbyRT—PeRResultITheexpressionofHIF—lawaspositiveinepitbelialovariancancerTumorburdeninsirolimusalone，Sirolimus

5、paelitaxe]，pac]itaxelaloneWaSreducedby4791％(pO05)respectirelycomparedwithcontrolS2TheexpressionofHIF—lQwasinhibitedbyrapamycin(siro]imus)(PO05)，TherewasasignificantcorrelationbetweenHIF一1aproteinexpressionandVEGF(byspear

6、man’Scoeffieientr=O8264，PO01)，MVD(r=O484，PO05)3Andapoptoticindexoftumoreell(P005)wasincreasedbysirolimus(rapamycin)comparedwithcontr01fPO05)whilethemRNAofbcl—2wasdecreasedConclusionRapamycinwhichinhibitexpressionofHIF一1Ⅱ