棉花BI-1(Bax Inhibitor-1)基因在生物和非生物脅迫中的功能分析.pdf

1、碩士學位論文論文題目棉花BI1（BaxInhibit1）基因在生物和非生物脅迫中的功能分析學科專業(yè)名稱發(fā)育生物學申請人姓名霍雪寒導師姓名張軍研究員論文提交時間2013年4月8號單位代碼10445學號2010021072分類號Q78研究生類型研究生類型全日制碩士全日制碩士山東師范大學碩士學位論文目錄中文摘要中文摘要...............................................................

2、.........................................................IABSTRACT................................................................................................................III第一章第一章文獻綜述文獻綜述.........................

3、................................................................................11BI1基因的研究........................................................................................................12植物啟動子研究進展...............

4、................................................................................32.1啟動子概述.............................................................................................................32.2植物啟動子的分類和應用.......

5、..............................................................................42.3啟動子克隆方法......................................................................................................92.4啟動子功能和結構研究的策略............

6、................................................................103本研究的目的和意義..............................................................................................11第二章第二章棉花棉花GhBI1基因在擬南芥中的功能分析基因在擬南芥中的功能分析.........

7、........................................131實驗材料..................................................................................................................131.1植物材料..................................................

8、..............................................................131.2菌株和載體............................................................................................................131.3主要試劑和酶.............................

9、..........................................................................131.4溶液及培養(yǎng)基的配制............................................................................................131.5儀器設備...............................

10、.................................................................................141.6引物序列................................................................................................................152實驗方法...........

11、.......................................................................................................152.1GhBI1A和GhBI1B轉基因擬南芥純合系的鑒定............................................152.2GhBI1A和GhBI1B轉基因擬南芥的脅迫處理實驗............

12、............................182.3GhBI1構建擬南芥AtBI1突變體的互補系......................................................193結果分析........................................................................................................

13、..........213.1GhBI1A和GhBI1B轉基因擬南芥的純合鑒定................................................213.2GhBI1A和GhBI1B轉基因擬南芥的脅迫處理實驗........................................233.3GhBI1在擬南芥atbi1突變體中的表達分析..................................

14、.................284討論與結論.............................................................................................................28第三章第三章棉花棉花GhBI1基因啟動子的克隆及瞬時表達分析基因啟動子的克隆及瞬時表達分析....................................301