腎細胞癌組織人端粒酶催化基團hTRTmRNA原位雜交研究.pdf

1、復(fù)旦大學碩士學位論文腎細胞癌組織人端粒酶催化基團hTRTmRNA原位雜交研究姓名：徐可申請學位級別：碩士專業(yè)：外科學指導(dǎo)教師：張元芳2001.5.1壅呈盔堂堡主墮塞生望些墮塞——ResearchOfHumanTelomeraseCatalyticSubunk(hTRTmRNA)inRenalCellCarcinomabyHybridizationinSituAbstractActivationoftelomeraseandstabili

2、zationoftelomeresalethoughttoberequiredforbothcellularimmortalityandoncogenesisThreemajorcomponentsofhumantelomerase，humantelomeraseRNA(hTR)，telomeraseassociatedprotein(TP1／TLP1)，andhumantelomerasecatalyticsubunit(hTRT／h

3、EST2)，havebeenidentifiedrecenetlyHoweveritremainsunclearwhatrolesthesesubunitsplayintheregulationoftelomeraseactivityInthepresentstudybyusingthemethodofhybridizationinsitu，humantelomerasecatalyticsubunitaledetectedin29ca

4、sesofrenalcellcarcinomaand30casesofnormalrenaltissueResultsshowedthatthepositiveexpressionpercentageofhTRTmRNAinrenalcellcarcinomaandnormalrenaltissueWaS862％(25／29)and63％(2／30)respectively111epositiveexpressionpercentage

5、ofhTRTmRNAWaSsignificantlyhigherthanthatofnomalrenaltissue(P001)Meanwhile，thecorrelationbetweenhTRTmRNAexpressionandclinicopathologicalcharacteristicssuch鵲tumorgradeclinicalstageandhistologicaltypeshowednostatisticalsign

6、ificanceThesetidingsindicatethattheupregulationofhTRTmRNAexpressionmayplayacriticalroleincarcinogenesisofhumanrenalcellcarcinomaandbeused船anewmarkerfordiagnosisofrenalcellcarcinomaKeywordsrella]cellcarcinoma；humantelomer