基因芯片研究肝硬化和原發(fā)性肝癌的基因表達譜.pdf

1、華中科技大學同濟醫(yī)學院碩士學位論文基因芯片研究肝硬化和原發(fā)性肝癌的基因表達譜姓名：黃海濤申請學位級別：碩士專業(yè)：消化內科指導教師：林菊生2002.5.1華中科技大學同濟醫(yī)學院碩士畢業(yè)論文GeneexpressionprofilingofcirrhosisandHepatcellularcarcinomawithcDNAmicroarraysThepathogenesisofhepatitisBvirus(HBV)associatedci

2、rrhosisandhepatocellularcarcinomainvolvesmanygenesfrommultiplepathogenicpathwayseDNAmicroarraycontaining1，568geneswasusedtocomprehensivelyanalyzethegeneticalterationsinHCCandlivercirrhosisAmongthe1，568targetgenes，therewe

3、re6676(42％48％)geneswhoseexpressionleveldifferedin2casesand28(18％)differentiallyexpressedgenesin3casesbetweenthenormalandcirrhotictissuesand19genesdifferentiallyexpressedbetweenthecirrhoticandHCCtissuesHierachicalclusteri

4、ngwasperformedtoidentifygenesrelatedtoclinicalphenotypesoftheHCCpatients12genesshowedchangesassociatedwiththedegreeofdifferentiationofHCCOurpresentanalysisclarifiedanumberofgenesthatcharacterizelivercirrhosisandHCCTheres

5、ultobtainedfrommicroarraymaybehelpfulindiagnosisforHBVassociatedcirrhosisandhepatocellularcarcinomaandinidentificationoftargetgenesfordesigningrationaltherapeuticstrategiesFurtheranalysisofthesedifferentiallyexpressedgen

6、eswillbehelpfulforunderstandingthemolecularmechanismoflivercirrhosis，itsmalignantchangeandclarificationofthemechanismofhepatocarcinogesisKeywords：eDNAmia[oim職hepatocellularcarcinoma；livercinhosis；differentlyexpressedgene